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当前位置: 首页 > 产品中心 > lipid > scarabgenomics/Electroporation Cuvettes, Sterile/Sterile, 1mm/D-0308-01
商品详细scarabgenomics/Electroporation Cuvettes, Sterile/Sterile, 1mm/D-0308-01
scarabgenomics/Electroporation Cuvettes, Sterile/Sterile, 1mm/D-0308-01
scarabgenomics/Electroporation Cuvettes, Sterile/Sterile, 1mm/D-0308-01
商品编号: D-0308-01
品牌: scarabgenomics
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 脂类
公司分类: lipid
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Background

Electroporation is commonly used to introduce nucleic acids to various cell types. Scarab Genomics Disposable Electroporation Cuvettes are designed for use with bacterial, yeast, fungi, and mammalian cells. Our superior quality standards will surpass any cuvette previously used. Our competitive pricing and quick turn around time will assist you in maintaining a highly efficient lab.

Figures

Figure 1: Sterile Disposable Electroporation Cuvettes. (A) The 1 mm gap cuvette is distinguished by its white cap. (B) The 2 mm gap cuvette has a blue cap.

Specifications

Kit Components Fifty individually wrapped gamma irradiated of either 1 mm or 2 mm gap cuvettes per bag Storage Conditions Store at room temperature.

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Support

Product Manuals Electroporation Cuvettes, Sterile Papers

  1. Pósfai G, et al., (2006) Emergent properties of reduced-genome Escherichia coli. Science 312:1044-6.

Patents & Disclaimers

Scarab is providing you with this Material subject to the non-transferable right to use the subject amount of the Material for your research at your academic institution. The Recipient agrees not to sell or otherwise transfer this Material, or anything derived or produced from the Material to a third party. NO RIGHTS ARE PROVIDED TO USE THE MATERIAL OR ANYTHING DERIVED OR PRODUCED FROM THE MATERIAL FOR COMMERCIAL PURPOSES. If the Recipient makes any changes to the chromosome of the Material that results in an invention in breach of this limited license, then Scarab will have a worldwide, exclusive, royalty-free license to such invention whether patentable or not. If the Recipient is not willing to accept the terms of this limited license, Scarab is willing to accept return of this product with a full refund, minus shipping and handling costs. For information on obtaining a license to this Material for purposes other than research, please contact Scarab’s Licensing Department. Scarab Genomics’ technology is covered by U.S. Pat. No. 6,989,265 and related foreign applications. Clean Genome® is a registered trademark of Scarab Genomics, LLC.

品牌介绍
scarabgenomics在pDNA生产中遇到的一个常见挑战是茎环DNA结构的存在,例如病毒长末端重复序列(LTR)或短发夹RNA(shRNA),它们难以复制并且即使在特意设计的E中也使载体不稳定大肠杆菌宿主,例如Stbl3™。清洁Genome® 大肠杆菌可显着提高不稳定pDNA载体的产量。CleanGenome®E . coli Out表现出用于慢病毒生产的Stbl3™。Chakiath&Esposito(2007)研究表明,“包含慢重复序列的慢病毒表达克隆通常在大肠杆菌中表现出极大的不稳定性。。” 即使是专门设计用于克隆慢病毒直接重复序列的宿主(例如Stbl3™)也被证明是不足够的。Chakiath&Esposito表明,CleanGenome® 大肠杆菌菌株MDS™42是基因组平台减少的基础菌株,可稳定包含这些重复序列的慢病毒表达克隆(图1)。在使用MDS™42进行的100多次克隆反应中,这些作者仅选择了两个转化子菌落进行分析,在超过95%的情况下,两个克隆都具有正确的限制性图谱,从而节省了重组质粒制备的大量时间和精力。同样,衍生自腺相关病毒(AAV)的载体带有两个反向重复序列,形成40 bp茎。在末端,另外两个9bp的茎分支进一步终止于环结构。这两个茎环本身包含直接重复序列,当在标准大肠杆菌宿主中生长时,特别容易被删除。极端的例子是pT-ITR-IL2载体(图2),其中包含ITR1和ITR2茎环,它们也是彼此直接重复的。为了测试CleanGenome®菌株解决不稳定的生物治疗性pDNA生产挑战的能力,圣甲虫基因组公司使用了腺相关病毒pT-ITR-IL2载体。将其转化到MDS™42和未还原的亲本菌株大肠杆菌中K-12 MG1655。选择含有对NotI,KpnI和MscI具有正确限制模式的质粒的克隆。为了测试在大肠杆菌中的生长过程中的稳定性,将来自两个宿主的一式三份的克隆在Luria Broth中在37°C下生长24小时,并进行选择。取出培养样品进行分析,然后将每组的一种正确培养物通过在新鲜肉汤中稀释10-6 – 10-7来开始连续传代。再进行四次相似的连续传代,从每个阶段分离质粒DNA以进行分析。在MDS™42中生长的pT-ITR-IL2的限制性酶切消化模式没有随传代而改变,而MG1655生长的质粒不稳定(图3)。)。KpnI消化物(单个位点)显示出较小的质粒片段,与通过内部重组使质粒重复序列之间的“锤头”区域丢失相一致。MscI消化(每个ITR两侧的位点)同样显示出片段之一的逐渐丢失,与KpnI消化一致。DNA测序证实了这些结果。